Plasma/serum Sample Preparation Kits
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Plasma preparation to measure FDA-approved protein markers by selected reaction monitoring
BACKGROUND The development of commercially available panels for human blood plasma screening via selected reaction monitoring (SRM) offers reliable, cost-efficient and highly-standardized discovery and validation of protein biomarkers. However, protein detection by SRM can be hampered by interfering peptide fragment ions. To estimate the influence of interference on protein detection, we perfor...
متن کاملComplement C3 in serum and plasma, as measured by radial immunodiffusion with four commercial kits.
Quantitation of the C3 component of complement by single radial immunodiffusion is subject to error because the C3 molecule has several antigenic determinants and anti-C3 sera differ in their specificity to these determinants. C3 was measured in plasma (ethylenediaminetetraacetate anticoagulant) and serum, in five normal individuals, by use of four such commercial kits. The effects of ethylened...
متن کاملA method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays
Brain-Derived Neurotrophic Factor (BDNF) has attracted increasing interest as potential biomarker to support the diagnosis or monitor the efficacy of therapies in brain disorders. Circulating BDNF can be measured in serum, plasma or whole blood. However, the use of BDNF as biomarker is limited by the poor reproducibility of results, likely due to the variety of methods used for sample collectio...
متن کاملPerformance Evaluation of Kits for Bisulfite-Conversion of DNA from Tissues, Cell Lines, FFPE Tissues, Aspirates, Lavages, Effusions, Plasma, Serum, and Urine
DNA methylation analyses usually require a preceding bisulfite conversion of the DNA. The choice of an appropriate kit for a specific application should be based on the specific performance requirements with regard to the respective sample material. In this study, the performance of nine kits was evaluated: EpiTect Fast FFPE Bisulfite Kit, EpiTect Bisulfite Kit, EpiTect Fast DNA Bisulfite Kit (...
متن کاملRapid and Non‐Phenol Extraction of Circulating and Exosomal RNA from Bodily Fluids
Norgen Biotek has developed various products (Plasma/Serum Circulating RNA Purification Kits and Exosome RNA Purification Kits) that are specifically optimized for purifying circulating or exosomal RNA (large RNA and microRNA) from plasma/serum or urine with the following advantages: 1) Simple and Fast Procedure with no lengthy phenol:chloroform extraction, filtration or precipitation 2) Scalab...
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